Learn how to use this product Highly efficient and sensitive enzyme
The enzyme is an ideal tool for two-step RT-qPCR. Maxima Reverse Transcriptase is so sensitive that it synthesizes cDNA from a wide range of input total RNA amounts from 1 pg to 5 µg at elevated temperatures of 50 to 65°C. Due to its high thermostability, the enzyme efficiently transcribes GC-rich RNA regions. Gene-specific primers can improve specificity. Complete the reverse transcriptase reaction in 15 to 30 minutes.

You can use Maxima Reverse Transcriptase for a broad range of applications:
Two-step RT-PCR
Two-step RT-qPCR
First-strand cDNA synthesis
Construction of full-length cDNA libraries
DNA labeling
Primer extension

For easy use, several complete kits are available that include all reagents needed for your PCR, including the Maxima Reverse Transcriptase:
Maxima First Strand cDNA Synthesis Kit for RT-qPCR contains Maxima Enzyme Mix, 5X Reaction Mix, and nuclease-free water.
Maxima H Minus First Strand cDNA Synthesis Kit contains Maxima H Minus Enzyme Mix, Oligo(dT)18 and Random hexamer primers, 5X RT Buffer, dNTP Mix, and nuclease-free water.
The Maxima H Minus Double-Stranded cDNA Synthesis Kit can be used to perform first- and second-strand cDNA synthesis reactions in the same tube. The kit contains all the necessary components.