| Cell type | Insect (Sf9, Sf21) |
| Chemically defined | No |
| Protein-free (>10kD) | Yes |
| Non-Animal Origin (NAO) | No |
| Glutamine | With L-Glutamine |
| Phenol Red | No |
This protein-free medium contains L-glutamine and supports higher baculovirus expression.
Most insect cell lines do not require weaning from serum-containing or serum-free media into Insect-XPRESS culture media. If adaptation is needed, we recommend an initial split ratio of 1:2 in the Insect-XPRESS medium followed by sequential splits of 1:5 until the culture adapts to the new medium. You should maintain a cell concentration above 3.0 x 105 cells/ml during the weaning process.
For cryopreservation of insect cells, the Insect-XPRESS™ can be mixed 50:50 with Cryoprotective Medium (Cat. No. 12-769E). This Cell Culture Media is produced according to cGMP and labeled "For Further Manufacturing" (FFM).