DNA can be easily removed during RNA isolation using enzymatic digestion. DNase I is mixed with a special buffer and added directly to the spin column where the nucleic acids are bound. After digestion, the column is washed, and clean, high-quality RNA is collected.
The reagents are free of RNase, which helps protect the RNA. This kit removes DNA very effectively, making it great for situations where even tiny amounts of DNA could affect results. It’s useful for making DNA-free RNA, DNase footprinting, Nick translation, or removing DNA when making cDNA.

Sample type/Starting material RNA samples contaminated with DNA

Concentration DNase I / 20 KU/µl

Preparation time 20 minutes (digestion only)

Extract RNA