Learn how to use this product
biotechrabbit™ 2X YourTaq Direct-Load Hot Start PCR Mix is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. YourTaq Hot Start PCR Mix shows excellent PCR specificity and sensitivity for various amplicons. The mix is resistant to PCR inhibitor carry-over, such as blood (up to 20%), Ethanol, or humic acid, enabling PCR amplification from DNA templates with carry-over of PCR inhibitors.
The 2X YourTaq Direct-Load Hot Start PCR Mix contains pure biotechrabbit™ YourTaq DNA Polymerase, sufficient buffer density for direct loading onto agarose gels, extremely high-quality dNTPs, and two dyes (blue and yellow) that separate during electrophoresis, allowing migration progress to be monitored. In addition, the mix is suitable for the amplification of GC-rich templates (up to 70%) when paired with the 5× PCR Enhancer (BR BR1900201).
Info: The recommended annealing temperature is 2°C above primer Tm (use gradient PCR to optimize the annealing temperature).
