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biotechrabbit™ Pfu DNA Polymerase is a highly purified thermostable recombinant proofreading DNA polymerase. biotechrabbit™ Pfu DNA Polymerase amplifies targets up to 3–4 kb in size and exhibits an approximately 10 times higher accuracy than Taq DNA polymerase.
The enzyme catalyzes the template-dependent nucleotide polymerization in the 5'→3' direction. Additionally, the 3'→5' exonuclease (proofreading) activity corrects nucleotide incorporation errors, thereby increasing the fidelity and accuracy of DNA polymerization. The enzyme produces blunt-end PCR products and has no 5'→3' exonuclease activity nor detectable reverse transcriptase activity.
For the most demanding applications, the supplied 5X PCR Enhancer can be added to improve results when using templates with GC-rich sequences and complex structures.
