Learn how to use this product biotechrabbit™ 1-Step RT-PCR Master Mix is designed to easily and efficiently perform reverse RNA transcription and PCR amplification of cDNA in one step. Only RNA template, primers, and PCR-grade water need to be added. The 20X RT-RI Blend contains a blend of an efficient thermostable reverse transcriptase and a proprietary Ribonuclease Inhibitor, ensuring high cDNA yields. The 2X 1-Step Master Mix contains unique Hot Start Taq DNA Polymerase, MgCl2, dNTPs, and stabilizers in an optimized buffer and provides high PCR product yields with minimal background even when using low-abundance and complex templates. PCR enhancers included in the mix allow efficient amplification of complex templates, including the GC or AT-rich sequences.