HEKstar 293 is a chemically defined, protein-free and animal component-free production medium developed specifically for stable processes in HEK293 cells. The medium is part of the HEKstar 293 Media System, combining a robust expression medium with optional feeding supplements for batch and fed-batch workflows within one consistent platform.

The formulation is optimized to support high viable cell densities and sustained productivity, helping achieve high recombinant protein yields from early development through to large-scale bioreactor production. HEKstar 293 supports reliable suspension adaptation of various HEK293 derivatives and stable growth kinetics with viabilities consistently above 90%.

Thanks to its broad formulation, HEKstar 293 is suitable for a wide range of HEK production processes, including virus packaging workflows and pilot-scale transient transfection workflows. The medium is supplied sterile-filtered, with L-Glutamine, and is intended for research use and further manufacturing only.