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This unique quantitative PCR mix has been around for decades. Even though the formulation remained the same for years, the patented hot-start enzyme Thermo-Start® DNA Polymerase and the proprietary reaction buffer keep delivering on the promise of high sensitivity and specificity. It is so popular that Thermo Scientific can't remove it from the range of offerings even if they want to.
The ABsolute qPCR SYBR mix with the SYBR Green intercalating dye comes in 1 x 5 ml vial and a separate 100 μL MgCl2 (1M).
The stringent chemically modified enzyme-based hot-start requires an incubation step of 15 minutes, making it ideal for gene expression studies.
