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The TaqManâ„¢ RNA-to-CTâ„¢ 1-Step Kit is designed for reliable reverse transcription and real-time PCR in a single workflow. The kit delivers accurate performance across a wide dynamic range and has been validated with TaqManâ„¢ Gene Expression Assays for straightforward experimental setup. Consistent results are achieved with a broad range of targets, including AT-rich, GC-rich, and long sequences. The one-step format reduces hands-on time and minimizes pipetting steps, helping to lower the risk of handling errors.
The kit provides high amplification efficiency across a wide range of RNA input concentrations, ensuring dependable real-time PCR performance over multiple orders of magnitude. Compared to earlier one-step RT-PCR master mixes, it offers improved dynamic range and sensitivity, with performance comparable to two-step workflows.
High sensitivity at low target concentrations has been demonstrated using synthetic RNA templates with defined copy numbers. Statistical analysis across multiple replicates confirms reliable quantification at low input levels, supporting detection of targets present at very low copy numbers.