| Format | Enzyme + Buffer |
| RNase inhibitor included | No |
| Hot-Start | No |
| High fidelity | No |
| Long distance (>5 kb) | No |
| Multiplex | No |
Learn more about this product
Thermo Scientific's Taq DNA Polymerase (recombinant) - 100 U is a highly thermostable recombinant DNA polymerase from the thermophilic bacterium Thermus aquaticus. It is supplied as 100 U.
The enzyme catalyzes DNA synthesis in the 5′→3′ direction. It has no detectable 3′→5′ exonuclease, or proofreading, activity and has low 5′→3′ exonuclease activity. Taq DNA Polymerase also exhibits deoxynucleotidyl transferase activity, which frequently adds extra adenines to the 3′ ends of PCR products.
The recombinant enzyme is identified for standard PCR with templates of 5 kb or shorter. The stated pack size and, where applicable, the low-concentration format distinguish this configuration within the recombinant Taq DNA Polymerase range.
Its catalytic profile combines 5′→3′ polymerase activity with absent detectable proofreading activity and low 5′→3′ exonuclease activity. The frequent addition of a terminal adenine to PCR products follows from the enzyme’s deoxynucleotidyl transferase activity. The product is a recombinant form of the enzyme.