| Format | Enzyme + Buffer |
| RNase inhibitor included | No |
| Hot-Start | No |
| High fidelity | No |
| Long distance (>5 kb) | No |
| Multiplex | No |
Learn more about this product
Thermo Scientific's Taq DNA Polymerase (native, without BSA) - 500 U is a highly thermostable native DNA polymerase from the thermophilic bacterium Thermus aquaticus. This 500 U format is supplied without BSA.
The enzyme catalyzes DNA synthesis in the 5′→3′ direction. It has no detectable 3′→5′ exonuclease, or proofreading, activity and has low 5′→3′ exonuclease activity. It also exhibits deoxynucleotidyl transferase activity, which frequently adds extra adenines to the 3′ ends of PCR products.
Native Taq DNA Polymerase is identified for amplification of bacterial DNA sequences homologous to sequences found in E. coli. The native enzyme format and the stated presence or absence of BSA distinguish this product from other Taq DNA polymerase configurations.
The combination of 5′→3′ polymerase activity, absent detectable proofreading activity and low 5′→3′ exonuclease activity defines the enzyme’s stated catalytic profile. The frequent addition of a terminal adenine is associated with its deoxynucleotidyl transferase activity.