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The ready-to-use Strep-Tactin®XT coated 96-well microplate is well-suited for ligand binding assays, immunoassays, enzyme assays, or diagnostic assays under high-throughput conditions. Due to the high specificity and affinity (nM-pM) of the streptavidin variant Strep-Tactin® XT, Strep-tag®II or Twin-Strep-tag® fusion proteins can be selectively and stably captured even from complex mixtures. Biomolecules of interest are presented to interacting partners in a uniform manner, resulting in reliable and reproducible assays.
The solid-phase, multi-well format is compatible with standard multichannel pipettes and automated plate readers. Twin-Strep-tag® fusion protein binding capacity determined with bacterial alkaline phosphatase (97 kDa) is 4 pmol for 200 µl incubation volume.