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Strep-Tactin®XT 4Flow® Starter Kit contains all necessary reagents required for purification and detection of Strep-tag®II and Twin-Strep-tag® fusion proteins. It provides a Strep-Tactin®XT 4Flow® column, the wash Buffer W, Buffer BXT, and Buffer XT-R for purification and Strep-Tactin® HRP for subsequent western blot detection.
Strep-Tactin®XT 4Flow® columns contain a 4% agarose coupled with the streptavidin variant Strep-Tactin®XT. Due to the broad pH compatibility, outstanding target purity (> 95%), excellent pressure stability, and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low-concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it particularly suitable for the purification of high-molecular-weight targets.
To reduce the waiting period during the sample and buffer application, the use of the application aid WET FRED is recommended.
