Learn how to use this product
Strep-Tactin® Superflow® is a resin that can be used for the purification of strep-tagged proteins <90 kDa via affinity chromatography. It consists of highly cross-linked agarose beads coupled to the streptavidin variant Strep-Tactin®. Strep-Tactin® binds to proteins fused to a Strep-tag®II or a Twin-Strep-tag®, enabling a straightforward purification of tagged proteins reaching a target purity of >95%. Due to the targeted elution, tedious titration steps are not necessary.
In addition, regeneration allows for the reuse of the resin, enabling multiple purification cycles.
Please note that biotinylated proteins also bind to Strep-Tactin® Superflow®. If biotinylated proteins are expected, BioLock should be used. Furthermore, as this resin is a 50% suspension, 2 ml of resin is required for packing a 1 ml gravity column.
