| Format | RT Enzyme + Buffer |
| RNase inhibitor included | Yes |
Learn more about this product
Thermo Scientific's RevertAid RT Kit - 500 rxn kit is a complete system for synthesizing first-strand cDNA from mRNA or total RNA templates. The kit uses RevertAid Reverse Transcriptase, which has lower RNase H activity than AMV reverse transcriptase and remains active at 42–50°C. It supports synthesis of cDNA up to 13 kb, with an optimum reaction temperature of 42°C.
The supplied recombinant RiboLock RNase Inhibitor protects RNA from degradation at temperatures up to 55°C. First-strand cDNA generated with the kit can be used directly as a template in PCR or real-time PCR. It can also serve as the first step in second-strand cDNA synthesis or linear RNA amplification.
The system supports construction of full-length cDNA libraries and antisense RNA synthesis. Radioactively or non-radioactively labelled nucleotides may be incorporated into first-strand cDNA for use as probes in hybridization experiments, including microarrays. This configuration provides reagents for 500 reactions. All components required for the reverse-transcription reactions are included in the kit.