| Format | Phusion HF Buffer |
| RNase inhibitor included | No |
| Hot-Start | - |
| High fidelity | - |
| Long distance (>5 kb) | - |
| Multiplex | - |
Learn more about this product
Thermo Scientific's Phusion HF Buffer Pack, Detergent-Free contains four 1.5 mL tubes of detergent-free HF Buffer for use with Phusion DNA Polymerase. The stated error rate of the polymerase in HF Buffer is 4.4 × 10⁻⁷, lower than the 9.5 × 10⁻⁷ stated for GC Buffer. HF Buffer is consequently described as the default buffer for high-fidelity amplification.
The detergent-free formulation is intended for situations in which DNA templates need to remain free of detergents. Microarray and DHPLC applications are specifically cited as examples where detergent-free reaction buffers are recommended. GC Buffer may improve performance with certain difficult or long templates, including GC-rich DNA and templates with complex secondary structures, but this pack provides the detergent-free HF formulation. The product is supplied as four separate 1.5 mL tubes.