Phusion GC Buffer Pack - 4 x 1.5 mL

- SKU TH F-519L

Thermo Fisher's Phusion GC Buffer Pack contains four 1.5 mL tubes of GC Buffer for amplification with Phusion DNA Polymerase. GC Buffer is described as an alternative to HF Buffer for certain difficult or long templates, including GC-rich templates and DNA with complex secondary structures. The stated polymerase error rate in GC Buffer is 9.5 × 10⁻⁷.

SKU TH F-519L

€ 35,34 excl tax

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Features

Format	Phusion GC Buffer
RNase inhibitor included	No
Hot-Start	-
High fidelity	-
Long distance (>5 kb)	-
Multiplex	-

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Thermo Scientific's Phusion GC Buffer Pack contains four 1.5 mL tubes of GC Buffer for use with Phusion DNA Polymerase. GC Buffer is described as an alternative reaction buffer that can improve polymerase performance with some difficult or long DNA templates. Examples include GC-rich templates and templates that contain complex secondary structures. The stated error rate of Phusion DNA Polymerase in GC Buffer is 9.5 × 10⁻⁷, compared with 4.4 × 10⁻⁷ in HF Buffer. HF Buffer is therefore presented as the default option for high-fidelity amplification, while GC Buffer can be selected when challenging template characteristics affect amplification performance. For applications requiring DNA templates to remain free of detergents, detergent-free reaction buffers are recommended. This product supplies the standard GC Buffer formulation as four individual 1.5 mL tubes.