Learn more about this product
Thermo Scientific's Phusion Blood Direct PCR Master Mix - 500 x 20 µl rxns is a direct PCR master mix for amplification of DNA from whole blood. It removes the need for a separate DNA purification step before PCR. The formulation uses a modified Phusion Hot Start II High-Fidelity DNA Polymerase. This enzyme is resistant to the main PCR inhibitors present in blood and retains activity when blood accounts for as much as 40% of the PCR reaction. The supplied format provides 500 x 20 µl rxns. The row-level source identifies whole blood as the sample type, direct amplification as the use and inhibitor resistance as a key property. It does not list separate sample-preparation reagents, purification components or accessory tools.