| Format | Master Mix |
| RNase inhibitor included | No |
| Hot-Start | Yes |
| High fidelity | No |
| Long distance (>5 kb) | No |
| Multiplex | No |
Learn more about this product
Thermo Fisher's Phire Plant Direct PCR Master Mix - 500 x 50 µl rxns is designed for direct amplification of DNA from plant samples without a separate DNA purification step. The master mix is based on Phire Hot Start II DNA Polymerase, an engineered enzyme containing a DNA-binding domain.
The polymerase is described as robust and tolerant of PCR inhibitors found in plant material, including complex polysaccharides and polyphenols. This formulation supports direct PCR from plant samples while avoiding a preliminary DNA purification stage.
The supplied format provides 500 x 50 µl rxns. The row-level source identifies the direct-PCR purpose, polymerase basis and examples of plant-derived inhibitors. It does not list sampling tools, extraction columns or other accessory components, so those items are not included in this description.