| Format | Enzyme + Buffer |
| RNase inhibitor included | No |
| Hot-Start | Yes |
| High fidelity | No |
| Long distance (>5 kb) | Yes |
| Multiplex | No |
Learn more about this product
Thermo Fisher's Phire Hot Start II DNA Polymerase - 1000 x 50 µL rxns is an enhanced hot-start DNA polymerase developed for routine and high-throughput PCR applications. The enzyme was created through protein engineering and contains a double-stranded DNA-binding domain.
This DNA-binding domain supports short extension times of 10–15 seconds per kilobase. It also improves product yields and provides twofold higher fidelity than Taq DNA polymerase. The enzyme is described as robust and capable of amplifying long DNA fragments with high yields.
The supplied format supports 1000 x 50 µL rxns. The source describes the polymerase as a hot-start PCR enzyme and provides its extension rate, yield characteristics and fidelity comparison. It does not identify additional master-mix components or accessory reagents for this enzyme-only format.