| Format | First-strand cDNA synthesis kit |
| RNase inhibitor included | Yes |
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Thermo Fisher's Maxima H Minus Double-Stranded cDNA Synthesis Kit - 10 rxns is a complete system for efficient preparation of double-stranded cDNA from total RNA or mRNA. First- and second-strand synthesis take place in the same tube, without intermediate organic extraction or ethanol precipitation.
The single-tube format shortens the procedure and helps maximize cDNA recovery. Premixed components reduce the number of pipetting steps required, and the full process can be completed in less than two hours. The kit supports synthesis of full-length double-stranded cDNA and includes the primers, controls and reagents required for residual RNA removal.
The resulting full-length, blunt-end double-stranded cDNA is suitable for cloning and double-stranded cDNA library construction. This pack contains sufficient reagents for 10 reactions. The complete kit format combines the required synthesis components in one 10-reaction package, supporting both first- and second-strand preparation without intermediate purification. The stated applications are full-length blunt-end cDNA synthesis for cloning and double-stranded cDNA library construction.