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Insect-XPRESS with L-Glutamine, RUO, 1L is an insect culture medium developed for commonly used insect cell systems. It is designed for the culture of sf9/sf21, High Five, and Drosophila cells and contains L-glutamine in a ready-to-use 1L format. The medium is both protein-free and serum-free, supporting defined culture conditions for suspension and adherent cells. This makes it relevant for work with insect cell cultures where serum-free and protein-free conditions are required. Insect-XPRESS is used for baculovirus propagation and recombinant protein expression. The medium is linked to high protein yields in these applications, while the RUO designation and 1L pack size provide clear context for its supplied format and use. The description remains focused on the specified cell types, culture formats, and expression-related applications.