| Optimized for | siRNA;miRNA;RNA;DNA |
In vivo-jetPEIĀ® is a highly efficient method for delivering nucleic acid in any animal model, including mice, rats, rabbits, llamas, monkeys, and more. It can be used to target a wide range of organs through both systemic and local administration. The injection method that is used will determine which organs are targeted. For example, intravenous injection mainly targets the lungs but also involves the liver, spleen, pancreas, kidney, heart, bladder, and artery.
In contrast, intraperitoneal injection involves the ovary, pancreas, diaphragm, uterus, and stomach. You can combine Cell-specific promoters with a local injection route to limit specific tissue or organ expression. In vivo-jetPEIĀ® is the optimal reagent for delivering various types of nucleic acid through most systemic and local injection routes. Moreover, it is a ready-to-use reagent with a straightforward protocol, saving time and budget for formulation.
The nucleic acid and in vivo-jetPEIĀ® complexes can be prepared in just two simple steps and are ready to be injected into the animal model within 15 minutes. The Scientific Support team of Polyplus is always happy to provide protocols tailored to your specific needs. In addition to being used for in vivo functional and proof-of-concept studies, in vivo-jetPEIĀ® has been chosen as a delivery vehicle for several drug development programs due to its reliability, safety, and efficacy. Several preclinical studies and clinical trials are underway for cancer therapy, vaccination, and immunization, all of which use the GMP-compliant quality of in vivo-jetPEIĀ®.
In vivo-jetPEIĀ® is a safe excipient for nucleic acid delivery. There is no induction of major pro-inflammatory cytokines or increase in sera levels of hepatic enzymes following administration of in vivo-jetPEIĀ®/nucleic acid complexes (Bonnet et al., (2008), Pharm Res 25:2978-82). In vivo-jetPEIĀ® is a powerful polymer-based reagent with unique properties. It condenses nucleic acid into stable nanoparticles in the provided complexation solution, which are small enough to efficiently diffuse within tissues and enter cells through endocytosis while protecting naked nucleic acids from degradation.
At the cellular level, in vivo-jetPEIĀ® enables both endosomal escape via the proton sponge mechanism (Akinc et al. (2005), J Gene Med 7: 657) and crossing of the nuclear membrane (Brunner et al. (2002), Mol Ther 5: 80). These properties make in vivo-jetPEIĀ® the non-viral vector of choice for in vivo nucleic acid delivery, as demonstrated by its use in over 600 peer-reviewed publications.