| Protein-free (>10kD) | Yes |
| Phenol Red | No |
| Non-Animal Origin (NAO) | Yes |
| Glutamine | Without |
| Chemically defined | Yes |
| Cell type | CHO |
CHO|ONE E is an expression medium suitable for the growth of recombinant Chinese Hamster Ovary (CHO) cells in small-scale shaker bottles, bioreactors, or spinner flasks. This CHO cell medium comes without Insulin & without L-Glutamine and is supplemented with Pluronic™. It can be used for the effective cultivation of recombinant CHO cells, achieving a high yield of the active protein.
CHO|ONE E is part of the CHO|ONE Media System™, which contains all media and supplements necessary for the growth of CHO cells in batch or fed-batch processes. The CHO|ONE Media System™ was invented for small- and large-scale protein production. CHO|ONE E not only leads to increased cell growth and protein yield but also, when combined with CHO|ONE Feed 1 and Feed 2, the full potential of the Media System can be deployed.
The CHO|ONE Media System™ was designed in close cooperation with a biopharmaceutical cooperation partner and is highly suitable for fed-batch cultures. It improves the yield and concentration of recombinant proteins by optimizing the selection of essential nutrients and growth-promoting factors.