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Bovine Serum Albumin (BSA), also "Fraction V," is a serum albumin protein derived from bovine blood. The BSA protein is purified and fractionated through a heat-shock procedure, followed by extensive diafiltration and ion exchange to remove other plasma proteins, lipids, and extraneous ions.

BSA Fatty Acid-Free (BSA FAF) has extremely low levels of total lipids and fatty acids, making it suitable for various applications in which the presence of lipids and fatty acids may interfere. BSA has widespread use in various biochemical applications, such as ELISAs (Enzyme-Linked Immunosorbent Assay), immunohistochemistry, and immunoblots.
Furthermore, it is used as a stabilizer and cell growth supplement in cell and microbial cultures. Further applications include, e.g., serum-free cell culture as an additive for hybridoma cell culture, protein stabilizer, and blocking agent.