Learn how to use this product biotechrabbit™ 2X YourTaq PCR Master Mix can be used for routine and demanding PCR amplifications and is optimized for a high amplification yield of 0.1–3 kb DNA targets, even from low copy numbers. The mix is exceptionally pure and shows excellent PCR specificity and sensitivity for various amplicons. The 2X YourTaq PCR Master Mix is resistant to PCR inhibitor carry-over, such as blood (up to 20%), Ethanol, or humic acid, enabling PCR amplification from DNA templates with carry-over of PCR inhibitors. The 2X YourTaq PCR Master Mix contains the pure biotechrabbit™ YourTaq DNA Polymerase, an optimized PCR buffer, and extremely high-quality dNTPs. Thus, only template, PCR primers, and PCR-grade water have to be added. In addition, the mix is suitable for the amplification of GC-rich templates (up to 70%) when paired with the 5X PCR Enhancer (BR BR1900201). Info: The recommended annealing temperature is 2°C above primer Tm (use gradient PCR to optimize the annealing temperature).