Learn how to use this product
biotechrabbit™ lyophilized 2X YourTaq Hot Start PCR Mix is a lyophilized (freeze-dried) version of the liquid equivalent. This stabilized format allows for storage and shipment without cooling. The Master Mix is optimized for a high amplification yield of 0.1–3 kb DNA targets, even from a low copy number, and shows excellent PCR specificity and sensitivity for various amplicons. The mix is resistant to PCR inhibitor carry-over, such as blood (up to 20%), Ethanol, or humic acid, enabling PCR amplification from DNA templates with carry-over of PCR inhibitors.
The 2× YourTaq Hot Start PCR Mix contains the pure Biotechrabbit™ YourTaq DNA Polymerase, an optimized PCR buffer, and extremely high-quality dNTPs. Thus, only template, PCR primers, and PCR-grade water need to be added. In addition, the mix is suitable for the amplification of GC-rich templates (up to 70%) when paired with the 5X PCR Enhancer (BR BR1900201).
Info: The recommended annealing temperature is 2°C above primer Tm (use gradient PCR to optimize the annealing temperature).
