Learn how to use this product
biotechrabbit™ YourTaq Hot Start PCR Master Mix is optimized for a high amplification yield of 0.1–3 kb DNA targets, even from low copy numbers. The mix shows excellent PCR specificity and sensitivity for various amplicons. The mix is resistant to PCR inhibitor carry-over, such as blood (up to 20%), Ethanol, or humic acid, enabling PCR amplification from DNA templates with carry-over of PCR inhibitors.
The 2X YourTaq Hot Start PCR Master Mix contains pure biotechrabbit™ Hot Start Taq DNA Polymerase, optimized PCR buffer, and extremely high-quality dNTPs. Thus, only template, PCR-grade water, and PCR primers need to be added. In addition, the mix is suitable for amplification of GC-rich templates (up to 70%) pairing with 5X PCR Enhancer (BR BR1900201).
Info: The recommended annealing temperature is 2°C above primer Tm (use gradient PCR to optimize the annealing temperature).
