| Detection Chemistry | Probe |
| Application | One-Step qRT-PCR |
| Colored Mix | Yes |
| Reference dye | ROX |
Learn more about this product
Thermo Fisher's Verso 1-Step QRT-PCR Low ROX Kit 400 rxns is formulated for one-step RT-qPCR using probe chemistry and a low ROX reference dye concentration. Reverse transcription and real-time PCR are carried out in the same tube, reducing sample handling and the number of transfer steps.
The kit uses Verso reverse transcriptase, which operates across 42–57°C and is designed to transcribe RNA through difficult secondary structures. The stated detection range is 1 pg to 1 µg RNA. An optional RT Enhancer can be included during reverse transcription to limit genomic DNA carry-over without a separate DNase I treatment.
An inert blue dye increases contrast between the reagent and plasticware, making master-mix dispensing easier to verify. The kit is intended for gene-expression analysis, RNAi validation, microarray validation, pathogen detection, genetic testing and disease research, as listed in the product information.
This Low ROX configuration is supplied for 400 reactions.
The one-step format is intended for experiments in which the generated cDNA does not need to be stored for later use.