Learn how to use this product
Biotechrabbit™ Taq DNA polymerase is a first-choice enzyme for all routine and molecular diagnostic PCR applications. The enzyme has exceptional quality and purity, ensuring the highest performance utilized by the diagnostics industry and research labs. The polymerase is well-suited for standard and fast PCR applications, giving high product yields from various templates with targets of up to 3 kb in size.
Taq DNA polymerase is a thermostable, highly processive 5'→3' DNA polymerase with low 5'→ 3' exonuclease activity and lacks 3'→5' exonuclease (proofreading) activity. The latter enables the incorporation of modified nucleotides.
The enzyme also exhibits deoxynucleotidyl transferase activity that adds extra A overhang at the 3'-ends of PCR products, allowing easy cloning of PCR products into vectors with T overhangs.
Info: The recommended annealing temperature is 2°C above primer Tm (use gradient PCR to check).
