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SF Cell Line 384-well-Nucleofector Kit is one of three kits for high-throughput transfection of cell lines when working with the 384-well HT Nucleofector System. It is used in the 384-well Nucleofector format and is available in variants for either 2 x 384 or 10 x 384 reactions. The kit has been determined to be optimal for a broad selection of cell types, including 293-F, A549, CHO-K1, CHO-S, HEK293, HepG2, HL-60, HT-29, K-562, LNCaP, Nalm-6 and other cell lines. Cell type-specific Optimized Protocols or recommendations are available through the relevant instructions or knowledge database. These protocols indicate Nucleofection conditions and provide information for treatment of individual cell types.