| Type | RPMI |
| Phenol Red | Yes |
| Glutamine | Without |
Roswell Park Memorial Institute (RPMI) 1640 is a growth medium used in different cell culture applications. This formulation of RPMI 1640 comes without L-Glutamine and is supplemented with 25 mM HEPES. RPMI 1640 medium is suitable for supporting the growth of many cell types and was developed to support lymphoblastoid cells in suspension cultures. It can support a wide range of other cell types, such as HeLa, MCF-7, PC12, Jurkat, PBMC, astrocytes, and carcinomas.
Originally intended to be supplemented with serum, RPMI 1640 has also been shown to support several cell lines in the absence of serum. Compared to other media, RPMI 1640 has a unique formulation containing glutathione and high concentrations of vitamins. Liquid media provide basic nutrients for all cell culture applications.
Each of Capricorn Scientific's™ high-quality cell culture media is manufactured according to the originally published formula or following modifications essential for consistent performance and stability of the culture medium.