| Nucleofector system | 384-well |
| Target cells | Range of primary cells |
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Primary Cell Optimization 384-well-Nucleofector Kit is used for high-throughput optimization of transfection conditions for primary cell types lacking an Optimized Protocol for the 384-well HT Nucleofector System. Different conditions can be tested within one experiment using a 384-well Nucleocuvette Plate and pmaxGFP Vector as a positive control.
The kit uses the five Primary Cell Nucleofector Solutions P1-P5 in combination with a pre-selected set of programs and controls. An optional fine tuning step can be performed with support from the scientific support team.
The kit also supports cell group-specific optimization using Basic Protocols. These protocols refer to a pre-selected set of one or two Primary Cell Nucleofector Solutions and Nucleofector Programs for groups including endothelial cells, epithelial cells, fibroblasts, glial cells, neurons, smooth muscle cells and human stem cells.