Cell type | Lymphocyte |
Protein-free (>10kD) | - |
Phenol Red | Yes |
Non-Animal Origin (NAO) | No |
Glutamine | With L-Glutamine |
Chemically defined | No |
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Lymphocyte Separation Medium (LSM)
Lymphocyte Separation Medium (LSM) is a sterile, ready-to-use reagent for the in vitro isolation of lymphocytes and monocytes from whole human blood, bone marrow, buffy coats, and other starting materials. The separation solution contains Ficollâ„¢, a hydrophilic polymer with a molecular weight of 400000 Dalton. It is used for the production of density gradients for the separation of cells and sub-cellular components, which sediment during centrifugation due to gravity.