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Luminaris Color Probe High ROX qPCR Master Mix - 5,000 x 20 µL rxns
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Thermo Fisher's Luminaris Color Probe High ROX qPCR Master Mix - 5,000 x 20 µL rxns is a 2X master mix for probe-based qPCR and two-step RT-qPCR. The formulation combines Hot Start Taq DNA Polymerase, uracil-DNA glycosylase, dNTPs including dUTP and an optimized PCR buffer with an inert blue dye. The pack supports 5,000 reactions at a 20 µL reaction volume. The master mix contains a high concentration of ROX passive reference dye. A separate 40X Yellow Sample Buffer and nuclease-free water are supplied. Combining the blue master mix with the yellow sample buffer produces a green reaction mixture, providing a visible aid during pipetting. The Hot Start Taq and optimized buffer support specific amplification, while the dUTP and UDG components provide carry-over contamination control. Template, primers and the selected sequence-specific probe are added separately. The formulation supports quantification of genomic, plasmid, viral and cDNA templates. Listed applications include gene expression analysis, siRNA validation, genotyping and pathogen detection, and standard cycling protocols can be used.