Learn how to use this product
biotechrabbit™ Long Range PCR Master Mix is designed for the amplification of targets up to 40 kb in size. It is the perfect choice for a fast reaction setup for long-range PCR that reduces the time required for calculation and pipetting and eliminates the need for buffer optimization. The master mix amplifies DNA with a higher fidelity than Taq DNA polymerase and works well with GC-rich templates.
The Long Range PCR Master Mix contains a blend of thermophilic polymerases, optimized PCR buffer, and extremely high-quality dNTPs. Thus, only template, PCR primers, and PCR-grade water need to be added.
The supplied 5X PCR Enhancer can be added for the most demanding applications to improve the results when using templates with GC-rich sequences and complex structures.
The Long Range PCR Master Mix produces a mixture of A-tailed and blunt-end PCR products. It is advisable to blunt products before cloning into the blunt-end vector.
