INNUSCREEN innuSPEED Soil DNA Kit 2.0 is a tool for extracting microbial DNA from soil samples

The extraction combines rapid homogenization of a highly complex starting material with efficient digestion of microbial cell walls in specially designed Lysis Tubes (using a SpeedMill, Retsch mixer mill, or other homogenizer). After mechanical lysis, followed by thermal lysis, the DNA is selectively bound to the surface of a spin filter membrane. A novel wash buffer efficiently removes inhibitors such as humic acids. Finally, DNA is eluted from the membrane using a low-salt buffer.

Sample type/Starting material: Soil

Extraction time: Homogenization and lysis: 30 minutes

Purification: approx. 16 minutes

Average yield: Depends on the type and quantity of microorganisms in the soil. Up to 30 µg

Starting material: Soil samples (400 mg)

Binding capacity: Column binding capacity: > 30 µg DNA

Average purity: 1,7 – 2,0

Extract: DNA