In the last few years, the importance of the cell-free miRNAs is constantly increasing

Mature cell-free microRNAs (miRNAs) are a class of naturally occurring , small, non-coding RNA molecules, about 18–25 nucleotides in length. They can circulate in blood inside of microvesicles (MVs) or as protein complexes.

Intended use of Innuscreen innuPREP Micro RNA Kit

The innuPREP cell-free microRNA Kit has been designed as a tool for very fast and efficient isolation of cell-free microRNAs from exosomes and other MVs isolated. It is possible to combine this kit with the use of innuPREP

PME Exosome Enrichment Kit, ultracentrifugation, filtration, and similar methods. InnuPREP cell-free microRNA Kit can also be successfully used for isolation of microRNA from unprocessed samples, exosome-depleted supernatant obtained after exosome separation by the above-mentioned techniques, or from samples sized by size exclusion chromatography.

The extraction procedure is based on a new kind of chemistry that combines a fast lysis step with efficient subsequent binding of microRNA to a Spin Filter surface, followed by washing of the bound microRNA and final elution of the microRNA.The whole protocol for the extraction of cell-free miRNAs requires approx. 60 minutes.