Sample type/Starting material of innuPREP DOUBLEpure Kit

PCR reactions
Agarose gel (TBE or TAE)

Starting material
PCR reaction mixes (up to 50 µl)
TAE agarose gels (up to 300 mg)
TBE agarose gels (up to 300 mg)
Sequencing reaction mixture (up to 50 µl)

Average recovery rates
PCR-purification: ca. 60 % – 95 %
Gel extraction: ca. 60 % – 90 %
Sequencing reaction purification: > 75 %
Depending on fragment length

Extraction time
PCR purification: approx. 3 minutes (2-step process)
Gel extraction: approx. 20 minutes
Sequencing reaction purification: approx. 2 minutes (2-step process)

Fragment lengths
PCR-purification: > 60 bp – 30 kb
Gel extraction: 100 bp – 30 kb

Binding capacity 
Column binding capacity: > 20 µg DNA

Extract DNA