Learn how to use this product
biotechrabbit™ 2X Hot Start PCR Master Mix lyophilized (freeze-dried) can be used for low-background, high-throughput PCR of 0.2-3 kb DNA targets. It is a perfect choice for a fast reaction setup that reduces the time required for calculation and pipetting and eliminates the need for buffer optimization.
The 2x Hot Start PCR Master Mix contains pure biotechrabbit™ Hot Start Taq DNA Polymerase, optimized PCR buffer, and extremely high-quality dNTPs. Thus, only template, PCR-grade water, and PCR primers are added.
The Hot Start Taq DNA Polymerase is inactive during reaction setup due to the bound antibody, which is quickly released at elevated temperatures. This ensures that the enzyme is only active during PCR. There is no need for prolonged heating or denaturation steps. The hot start minimizes primer–dimers and mispriming.
Info: The recommended annealing temperature is 2°C above Tm of primers, or gradient PCR can be used to optimize the annealing temperature.
