Hanks' Balanced Salt Solution (HBSS) without Ca, Mg, and Phenol Red, is a buffered salt solution.

It can be used in various cell culture applications, such as rinsing cells, transporting cells or tissue samples, and diluting cells for counting.

Functions of Hanks' Balanced Salt Solution (HBSS)

The essential functions of a balanced salt solution are to maintain pH and osmotic balance and to provide cells with water and essential inorganic ions. When using HBSS as a buffer, it can neutralize small amounts of acid or base and act as a stabilizer to maintain the physiological pH level of a solution.