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Hanks' Balanced Salt Solution (HBSS) without Ca, Mg, and Phenol Red, is a buffered salt solution. It can be used in various cell culture applications, such as rinsing cells, transporting cells or tissue samples, and diluting cells for counting.

The essential function of a balanced salt solution is to maintain pH and osmotic balance and provide cells with water and essential inorganic ions. When using HBSS as a buffer, it can neutralize small amounts of acid or base and act as a stabilizer to maintain the physiological pH level of a solution.