| Detection Chemistry | Green Intercalating Dye |
| Application | QPCR |
| Colored Mix | No |
| Reference dye | ROX |
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Thermo Scientific's DyNAmo Flash SYBR Green qPCR Kit - 2,500 x 20 µL is developed for fast real-time qPCR using SYBR Green detection. The kit is also stated for RT-qPCR with SYBR Green dye.
A DNA-binding domain attached to the polymerase improves the physical stability of the polymerase–DNA complex, supporting processivity and robustness. The protocol uses a combined annealing and extension step of 15 seconds and is designed for specific and sensitive detection across a wide range of template concentrations. High amplification efficiency supports reliable quantification and early Cq values, while high signal intensity provides a strong signal-to-noise ratio.
The convenient 2X master mix includes dUTP, allowing UNG to be used for carry-over contamination control. The supplied format provides 2,500 x 20 µL.