| Detection Chemistry | Green Intercalating Dye |
| Application | QPCR |
| Colored Mix | Yes |
| Reference dye | ROX |
Learn more about this product
Thermo Fisher's DyNAmo ColorFlash SYBR Green qPCR Kit - 500 x 20 µL is a SYBR Green qPCR kit with a multicolor system for monitoring pipetting during reaction setup. The 2X master mix contains a blue dye, while a separate sample buffer contains a yellow dye. When both components are present, the reaction mixture appears green.
This color change allows addition of the master mix and sample to be checked visually, particularly when white reaction vessels are used. The dyes are stated not to affect qPCR specificity or sensitivity.
The kit supports fast qPCR and RT-qPCR using SYBR Green dye. Its protocol uses a combined annealing and extension step of 15 seconds and is designed for specific and sensitive detection across a wide range of template concentrations. dUTP is included in the 2X master mix, allowing UNG to be used for carry-over contamination control. The supplied format provides 500 x 20 µL.