| Type | SSC buffer |
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AccuGENE 20X SSC Buffer 1L is a 20X SSC buffer supplied in a 1 L format. SSC, or saline-sodium-citrate, is used as a hybridization buffer and to control stringency during washing steps.
The buffer is used in protocols for Southern blotting, in situ hybridization, and Northern blotting. It may also be used to prevent drying of agarose gels during a vacuum transfer.
The formulation is 3.0 M NaCl and 0.3 M sodium citrate at pH 7.0.