Learn how to use this product
The Twin-Strep-tag® Capture Kit is intended for site-directed, reversible capture of Twin-Strep-tag® fusion proteins for biomolecular interaction analysis using surface plasmon resonance (SPR) devices. The kit includes Strep-Tactin®XT, Immobilization Buffer, Regeneration Buffer, and GFP-Twin-Strep-tag® as a control protein.
The kit enables the highly stable immobilization of the ligand based on the unique Strep-Tactin®XT:Twin-Strep-tag® interaction, which operates with pM affinity.
The key benefits include the protection of the ligand from chemical modification as well as inactivation or manipulation by the immobilization procedure through indirect immobilization. The high affinity enables measurements over a long period (high affinity in low pM range; T1/2 = 13 h), and the chip can be regenerated easily by an established cost-effective procedure.
Lastly, ligands can be captured directly from complex samples due to the high affinity and specificity of the Twin-Strep-tag®:Strep-Tactin®XT interaction.
The use of unconjugated Strep-Tactin®XT requires a license, which is provided with the purchase of the kit as a limited-use license.
