Learn how to use this product biotechrabbit™ T4 DNA Ligase Rapid is a highly concentrated and exceptionally pure ligase for applications that require high enzyme concentrations. It is especially well-suited for fast ligations. T4 DNA Ligase Rapid is supplied with a 5X Rapid Ligation Buffer containing PEG for fast (5–10 minutes), low-concentration, or blunt-end DNA ligation. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5' phosphate and the 3' hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single-stranded nicks in duplex DNA, RNA, or DNA–RNA hybrids.