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The RTS pIVEX Wheat Germ vectors are designed for high-yield cell-free expression of His6-tagged eukaryotic proteins using the RTS Wheat Germ kits.
The two vectors contain all regulatory elements necessary for in vitro expression based on a combination of T7 RNA polymerase and wheat germ lysates. They enable the attachment of a His6-tag in different orientations at the C- and N-terminus of the protein. Cloning into RTS pIVEX Wheat Germ His6-tag vectors promotes optimal protein expression in RTS Wheat Germ CECF kits to produce more than 1 mg protein per 1 ml synthesis volume in a 24-hour reaction.
