Learn how to use this product
The Rapid Translation System (RTS) is a cell-free protein expression system in which reaction conditions can easily be adapted in a protein-specific manner by adding cofactors, ligands, detergents, or chaperones to the reaction mixture.
The biotechrabbit™ RTS DnaK Supplement includes DnaK (heat shock protein (hsp) 70), one of the key chaperone systems in E. coli, in combination with DnaJ (hsp 40) and GrpE. The RTS DnaK Supplement can be used to increase the amount of correctly folded and/or soluble proteins expressed in the RTS.
DnaK can be used with proteins of any size and serves as an aggregation inhibitor that binds unfolded protein or transfers the protein to other chaperones, such as the GroE or the Hsp90 systems. The RTS DnaK Supplement can be used with the RTS E. coli HY platform.
