Learn how to use this product The biotechrabbit™ RTS 9000 E. coli HY Kit provides a reaction volume ten times higher than the RTS 500 E. coli HY Kit while maintaining a similar productivity per milliliter of lysate and can yield ten times more total protein (up to 50 mg) than an RTS 500 reaction. The RTS 9000 Reaction Device comprises a reaction compartment and a feeding compartment. The 10 ml reaction compartment, where the coupled transcription/ translation reactions occur, is separated from the 100 ml feeding compartment by a semipermeable membrane (cut-off 10 kDa).