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The biotechrabbit™ RTS 100 Wheat Germ Kit is a batch screening system that allows rapid protein expression within 2.5 hours. The kit enables the use of a wide range of nonoptimized T7 promoter-based expression vectors from eukaryotic and prokaryotic origin in addition to established cell-free wheat germ vectors or linear templates. Using this approach, multiple constructs, including vectors that have not been optimized for wheat germ, can be screened rapidly for expression and functionality in parallel. The procedure saves precious time otherwise required for template generation. The new batch system yields up to 100 μg protein per ml reaction, sufficient for many downstream applications.
